Analysis of Nivalenol and Deoxynivalenol in Wheat Using Prominence-i

Nivalenol and deoxynivalenol (DON, vomitoxin) are types of mycotoxins produced by Fusarium fungi. In Japan, the provisional reference value for deoxynivalenol was set at 1.1 ppm in May, 2002 (Notification No.0521001 issued by Department of Food Safety, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare of Japan). Previously, in Application News No. L362, the analysis using an ultra high-performance LC system was introduced, but here, referring to the test method for deoxynivalenol specified in the Notification No. 717001, a washing process for the analytical column is included.