Comprehensive 2D Separation of Carotenoids in Red Chili Pepper by the Nexera-e System

Carotenoids are rich in double bonds, and therefore have received much attention in recent years as antioxidants, which are known for their disease preventive properties, including lifestyle-related diseases. The extensive range of carotenoids found in foods makes it difficult to conduct simultaneous separation and quantitation by conventional HPLC. However, the Nexera-e comprehensive two-dimensional LC is particularly suited for such analyses.
Here, carotenoids extracted from red chili pepper were subjected to two-dimensional analysis, in which micro-scale separation was conducted in the first stage using normal phase conditions, and separation using reversed phase conditions was tried in the second dimension.