Optimization of Analytical Conditions, and Scale-up from Analytical to Preparative Scale Using Supercritical Fluid Chromatography

High-purity fractionation requires proper separation between peaks, and in order to achieve this, both an exhaustive investigation of conditions and the optimization of analytical conditions (method scouting) are important. Particularly with supercritical fluid chromatography (SFC), retention behavior changes substantially depending on the stationary phase, so it is useful to investigate conditions with a variety of different columns. Normally, optimal conditions are verified at the analytical scale by method scouting, and are then scaled up to the preparative scale by increasing flow rate and injection volume in proportion to the column cross-sectional area.
This report describes an investigation of conditions on a Nexera UC (analytical scale) system, after which the method obtained was scaled up for a Nexera UC Prep (preparative scale) system.