Development of Automated Screening and Quantitation Approach on Novel On-Line SFE-SFC-MS/MS Platform - (II) For Five Aflatoxins in Powdered Food Samples

Supercritical fluid chromatography (SFC) is one of the trendy analytical techniques in recent years. By using carbon dioxide (CO₂) as eluent, the SFC has a number of advantages like environmental friendly, cost effective and suitable for a wide range of analytes’ polarities. The Nexera UC system (Shimadzu) is a further developed platform combining SFE (extraction) and SFC with MS/MS into a complete system to handle from sample pre-treatment and separation to superior MS/MS detection and quantitation in a fully automated manner. The novel SFE-SFC-MS/MS system has been used successfully for analysis of 510 residual pesticides in agricultural products. Here we describe the applications and advantages of the Nexera UC system in analysis of aflatoxins (B1, B2, G1, G2 and M1) in powdered food such as corn flour and wheat flour.