Simultaneous Analysis of Water-soluble and Fat-soluble Vitamins in Fish by Novel SFC-MS/MS Method

Vitamins, essential dietary nutrients, are principally classifid into water-soluble and fat-soluble groups based upon the solubility. Multiple chromatographic systems are commonly employed to analyse water-soluble and fat-soluble vitamins separately as both groups exhibit different chemical properties. Supercritical fluid carbon dioxide (SF-CO2) has been extensively utilized as a common solvent due to its advantages including non-toxic, low viscosity, high diffusivity, and easily removed from the reservoir. Despite its nonpolar characteristic, the polarity of SF-CO2 can be easily attuned by adding polar solvents such as methanol and ethanol. With the use of SF-CO2 as the mobile phase, supercritical fluid chromatography - mass spectrometry (SFC-MS) has attracted much attention for separation and analysis of diverse compounds. By changing the composition of mobile phase from supercritical to near liquid phase with addition of polar modifier (e.g., MeOH) up to 90%, we develop a novel SFC-MS/MS method for simultaneous analysis of 17 water soluble and fat soluble vitamins in fish samples.