LCMS Bioanalysis of Antibody Drugs Using Fab-selective Proteolysis "nSMOL Method" -Selection of Signature Peptide -

nSMOL (nano-surface and molecular-orientation limited proteolysis) is Shimadzu's completely new, proprietary, and innovative technique for selective proteolysis of Fab region of monoclonal antibodies. nSMOL allows analytical method development of antibody drugs independent of a variety of antibody drugs. Fab-derived peptide fragments produced by nSMOL can be precisely quantified by multiple reaction monitoring (MRM) using the Shimadzu LCMS-8050/8060 triple quadrupole liquid chromatograph mass spectrometer (TQ-LCMS). This report describes a selection protocol of signature peptides suitable for pharmacokinetic studies.

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Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
nano-surface and molecular-orientation limited proteolysis, antibody drug, bioanalysis, LC/MS/MS, Pharmaceutical, Life Science, Proteomics, LCMS-8050, LCMS-8060, nSMOL Antibody BA Kit
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