High-throughput comprehensive analysis of trace D- and L- amino acids using extra-facile chiral separation and column switching

Amino acids (except for glycine) have a chiral carbon atom adjacent to the carboxyl group and form two enantiomers that are mirror images of each other. L-Amino acids are present in the body as a component of proteins and nutrients in large quantities. On the other hand, D-Amino acids are extremely low, but it is drawing attention in various fields such as ingredient analysis of fermented foods, physiological function analysis in the cranial nervous system and biomarker search, as well as health and beauty. Here we developed the method that be possible to analyze chiral amino acids with high sensitivity in just 10 minutes using a chiral column without derivatization and confirmed ratio of L/D amino acids in fermented foods.