Development of online SFE-LC/MS system for analysis of metabolites in microbial cells

In bio-based fine chemical production, a short breeding cycle time of microbes is a key issue for improving productivity. Metabolomics has been widely used as a quick and comprehensive analytical procedure due to its excellent features of dynamic monitoring and quick evaluation for bio-production. On the other hand, sample pretreatment procedure is still generally tedious and time consuming while high-throughput analytical methods using LC/MS and GC/MS. We present the development of online SFE-LC/MS system that provides completely automated analytical process resulting labor-saving.

Content Type:
Paper
Document Number:
PO-CON1796E
Product Type:
Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
Keywords:
Nexera UC SFE system, LCMS-8060 triple quadrupole mass spectrometer, metabolite, microbial cells, Pharmaceutical, Life Science, Metabolomics, online SFE-LC/MS system
Language:
English
File Name:
jpo118024.pdf
File Size:
728kb

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