LC-MS/MS detection of fentanyl and related synthetic opioids in biological matrices
The potency of these compounds result in small concentrations being detected in real-world samples, thus requiring methods utilized by a forensic or clinical laboratory to be very sensitive. Hyphenated techniques such liquid chromatography-tandem mass spectrometry (LC-MS/MS) can achieve this needed sensitivity through multiple reaction monitoring (MRM). The Shimadzu LCMS-8060 features a heated dual ionization source (DUIS) coupled with a along with ultrafast MRM acquisition software and polarity switching for increased accuracy, sensitivity and robustness. DUIS combines electrospray ionization (ESI) and atmospheric-pressure chemical ionization (APCI) by using the ESI source and the corona needle used in APCI. The development process involved MRM optimization, MS source optimization and finally column selection for a method that successfully separated and identified a mixture of synthetic opioids. This analytical method when used in combination with a validated solid phase extraction procedure, achieved sub-ng/mL detection limits in blood and urine samples.
- Content Type:
- Paper
- Document Number:
- PO-CON1816E
- Product Type:
- Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
- Keywords:
- opioids, Clinical research, Forensics, Clinical research, LCMS-8060
- Language:
- English
- File Name:
- sio118047.pdf
- File Size:
- 468kb