LC-MS/MS detection of fentanyl and related synthetic opioids in biological matrices

The potency of these compounds result in small concentrations being detected in real-world samples, thus requiring methods utilized by a forensic or clinical laboratory to be very sensitive. Hyphenated techniques such liquid chromatography-tandem mass spectrometry (LC-MS/MS) can achieve this needed sensitivity through multiple reaction monitoring (MRM). The Shimadzu LCMS-8060 features a heated dual ionization source (DUIS) coupled with a along with ultrafast MRM acquisition software and polarity switching for increased accuracy, sensitivity and robustness. DUIS combines electrospray ionization (ESI) and atmospheric-pressure chemical ionization (APCI) by using the ESI source and the corona needle used in APCI. The development process involved MRM optimization, MS source optimization and finally column selection for a method that successfully separated and identified a mixture of synthetic opioids. This analytical method when used in combination with a validated solid phase extraction procedure, achieved sub-ng/mL detection limits in blood and urine samples.

Content Type:
Paper
Document Number:
PO-CON1816E
Product Type:
Liquid Chromatograph-Mass Spectrometry, Mass Spectrometry
Keywords:
opioids, Clinical research, Forensics, Clinical research, LCMS-8060
Language:
English
File Name:
sio118047.pdf
File Size:
468kb

View Article

For Research Use Only. Not for use in diagnostic procedures.

This page may contain references to products that are not available in your country. Please contact us to check the availability of these products in your country.

Top of This Page