Glyphosate (Gly) is one of the most widely used herbicides in the world. It acts as an enzyme inhibitor to stunt the growth and vitality of plants. The potential health effects on humans exposed to glyphosate have been a concern. Therefore, methods that analyze glyphosate and aminomethylphosphonic acid (AMPA), the main degradant of glyphosate, are highly desired. An LCMS glyphosate and AMPA quantitation method was therefore developed using a Shimadzu triple quadrupole mass spectrometer for research use.