UV TALK LETTER Vol. 18

In UV Talk Letter Vol. 17, we focused primarily on the principles and characteristics of fluorescence. Here, we describe the spectrofluorophotometers used in fluorescence spectroscopy, focusing on their configurations and the data they generate as compared to the data generated by UV-VIS spectrophotometers. Also described is how spectrofluorophotometers in particular can record multiple types of data, including excitation spectra, fluorescence spectra, and 3D spectra, and that data generated by each spectrofluorophotometer include a coefficient unique to that instrument, and recorded data must be corrected according to this coefficient.