UV-Vis-NIR Spectroscopy

• Environmental analysis: For water-quality testing, including the detection of nitrate and phosphate, as well as pollutant monitoring.
• Pharmaceuticals: For analysis in the product-development and quality-control stages of production, including determining drug-content uniformity, purity analysis, dissolution testing and assay validation.
• Food and beverage manufacturing: For quality control and color analysis of products, as well as additive quantification.
• Clinical research and academia: For routine absorbance measurements and kinetic studies.
• Chemical manufacturing: For concentration determination and quality control.
• Biotechnology: For DNA and protein analysis.

FAQ

What is the instrumentation of ultraviolet visible spectroscopy?

Ultraviolet visible (UV-Vis) spectroscopy is an analytical approach that relies on UV-Vis spectrophotometers. The instruments comprise a light source, a monochromator, a sample holder and a detector. Together, these components accurately measure absorbance of light via the light source (either a deuterium or tungsten lamp), which irradiates the sample, a monochromator, which selects the wavelength of light that passes through the sample, the sample holder and the detector itself.

What does a UV-Vis spectrophotometer measure?

A UV-Vis spectrophotometer measures the transmittance, absorbance and reflectance of liquids, solids, powders, and gases using light. By measuring the amount of light that enters a sample and comparing it to the incident light, UV-Vis spectrophotometers can analyse and record specific characteristics of the various molecules within the sample. UV-Vis spectrophotometers specifically measure the absorbance of light in the UV-Vis range (190–1100 nm). This makes UV-Vis spectrophotometers ideal for DNA and protein quantification, water-quality testing in environmental science and color analysis in food and beverage quality control.

What is the difference between UV-Vis-NIR spectroscopy and UV-Vis-NIR spectrophotometers?

The difference between UV-Vis-NIR spectroscopy and UV-Vis-NIR spectrophotometers is that the former refers to the technique that analyzes samples using light in the UV-Vis-NIR range, while the latter refers to the instrument or system used to perform spectroscopy. In other words, UV-Vis-NIR spectroscopy is the science of analyzing absorbance, transmittance and reflectance of liquids, solids, powders, and gases for analytical chemistry, while the UV-Vis-NIR spectrophotometers are the hardware that incorporates the light source, monochromator, sample holder and detector to arrive at the results of the analysis. Shimadzu’s UV-Vis-NIR spectrophotometers are renowned for performance, with high-resolution optics, intuitive software, innovative accessories and automated features like seamless data integration that ensure reliable results.