Chapter 2 introduces the detailed configuration of the LCMS instrumentation. It generally consists of a LC separating system, a mass analyzer and the LCMS interface API unit. Generation of gaseous ions is crucial in LCMS and there are several factors limiting the efficiency of this API process. In addition, there are many analytical parameters to take note when switching from LC to LCMS, for instance, the flow rate and the types of mobile phases compatible with MS. In this chapter, we discussed in detail the API processes, their limiting factors and the key parameters to take note when coupling LC and MS.
Liquid chromatography mass spectrometry (LC-MS) is an indispensable tool for quantitative and qualitative analysis in a wide range of fields, from pharmaceuticals and food science to environmental analysis.