Aroma and Metabolite Analysis Using GC-MS and LC-MS and Approach to Craft Beer Development

User Benefits

－The Smart Aroma Database™ and Smart Metabolites Database™ Ver. 2 can perform a comprehensive analysis of aroma and metabolite compounds. －The Multi-Omics Analysis Package can efficiently analyze GC-MS and LC-MS metabolomic data. －The LC-QTOF can perform attribute analysis of unidentified compounds.

Introduction

Craft beers are beers that are brewed in small batches and enjoyed around the world. Craft breweries use select techniques and ingredients, such as barley and hops, to create beers that are enjoyed for their unique and varied aromas and tastes. Beer flavor analysis is performed using various methods and is an essential process in the development and quality control of beers. In recent years, there has been growing interest in using analytical instruments, rather than conventional human sensory evaluations, to objectively evaluate food flavors and functions by comprehensively analyzing aromas and metabolite compounds. In this article, beers were analyzed using gas chromatography-mass spectrometer (GC-MS) and liquid chromatography-mass spectrometer (LC-MS). First, aroma compounds were analyzed using GC-MS. Next, a complementary analysis of metabolite compounds in common samples was performed using targeted metabolomics with GC-MS and LC-MS. Finally, attribute analysis (non-targeted metabolomics) was performed on unidentified metabolites in the samples, based on the determination of the exact mass using quadrupole time-of-flight LC-MS (LC-QTOF). The compounds that can be detected by GC-MS and by LC-MS differ, so using both techniques makes it possible to complement the analytical data. The resulting data was then used to develop new craft beers.

March 6, 2024 GMT