DMT-on Purification of Phosphorothioate Oligonucleotide using SHIMSEN Styra HLB SPE Cartridge

User Benefits

- A simple and straightforward workflow for purification of modified phosphorothioate oligonucleotide. - Stability of SHIMSEN Styra HLB SPE cartridge at high pH allows direct loading of deprotected oligonucleotide without removal of the highly basic deprotecting agent. - Stability of SHIMSEN Styra HLB SPE cartridge at low pH allows on-cartridge detritylation using acid.

Introduction

Oligonucleotide has been used in various applications, including genetic testing (e.g. primers for PCR) and therapeutics (e.g. antisense oligonucleotides and siRNA). These oligonucleotides are usually chemically synthesized using solid-phase synthesis and would require purification to remove impurities such as protecting groups and failure sequences. In this regard, the use of solid-phase extraction (SPE) cartridges offers a simple and efficient strategy for purifying oligonucleotides without the need for complex instruments or extensive processing. In this application, we present the procedure for DMT-on purification of synthetic phosphorothioate oligonucleotide using SHIMSEN Styra HLB SPE cartridge (P/N: 380-00855-10). Analysis of purified oligonucleotide was achieved on Shimadzu Nexera XS inert system using Shim-pack Scepter Claris C18-120 which features a bioinert coating on the inner surface of the column body, preventing absorption of oligonucleotides to the column surface and increasing analysis sensitivity.

March 20, 2024 GMT