Efficient Scouting of Chiral Separation Conditions Using LabSolutions MD

User Benefits

－ Supercritical fluid chromatography offers quicker separation of chiral compounds. － LabSolutions MD offers more efficient scouting of analytical conditions for new compounds. － Peak shapes of acidic and basic analytes can be improved by adding suitable additives to the modifier.

Introduction

Chiral compounds contain at least one asymmetric carbon, so they cannot be superimposed on their mirror image. Although HPLC is the dominant chromatographic technique used to separate chiral compounds, there is growing interest in chiral separation that uses supercritical fluid chromatography (SFC). SFC is normally performed using a mobile phase of supercritical carbon dioxide, which offers low polarity, low viscosity and high diffusivity. Polar organic solvents (modifiers) are then added to this mobile phase to control interactions between the analytes and the stationary phase. Chiral compounds are typically separated by HPLC under normal phase conditions, but the unique properties of SFC mentioned above offer advantages, such as higher separation speeds and lower organic solvent consumption, which reduce costs and lower the environmental impact. Therefore, the pharmaceutical sector uses SFC because it can provide rapid optical resolution in the synthesis of novel drugs. Finding the best column and mobile phase (modifier) combination for a given analyte species from the wide variety of chiral columns available is a very time-consuming and labor-intensive process, so there is demand for faster and more streamlined methods for scouting chiral separation conditions. This article describes a workflow for developing separation conditions for chiral compounds that use the Nexera UC chiral screening system and LabSolutions MD analytical method development software.

March 26, 2024 GMT