The analysis time can be effectively shortened because an excellent chromatographic resolution can be maintained even at higher flow rates than those typically used for HPLC.
Nexera UC
Supercritical Fluid Extraction / Supercritical Fluid Chromatograph
Supercritical Fluid Extraction/Chromatograph System
Preparative scale Nexera™ UC Prep is Here
Advances in column technology have led to a renewed interest in supercritical fluid chromatography (SFC), which uses a supercritical fluid as mobile phase. Compared to liquid, supercritical fluids have low viscosity and high diffusivity. By combining SFC with Supercritical fluid extraction (SFE), which utilizes the high permeability and diffusivity of supercritical fluids, the extraction and analysis of target components from solid samples can be automated. The new analytical technology “Unified Chromatography” improves the analytical workflow by integrating sample extraction, analysis by various separation modes, and high-sensitivity detection.
Features
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Separation results can be quickly obtained during method development by screening with both SFC and UHPLC. -
By using a fraction collector, small-volume fractionation is also possible (ex:1.5 mL vials). -
Target compounds are extracted from solid samples, and then automatically transferred to SFC. -
By using SFC, the consumption of organic solvent is reduced by 94.2%, compared to the normal-phase.
Movies
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SFE-ENANTIOSELECTIVE-SFC-QQQ MS FOR THE DETERMINATION OF CHIRAL PESTICIDES IN HEMP SEEDS
This webinar describes how to develop the analytical method for chiral pesticides in hemp seeds by using online SFE-SFC coupled to triple quadrupole mass spectrometry. Additionally, it will introduce the principle of the on-line SFE-SFC system and how eco-friendly it is.
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Nexera UC Supercritical Fluid Extraction/Chromatograph System Narration Eddition
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Overview of the analytical technologies using supercritical fluid
Green Analytical Chemistry (GAC) is a concept of designing analytical methods that are friendly to both chemists and environment. It is necessary for the GAC-based methods to retain the same analytical performance (e.g., accuracy, sensitivity) as those by their conventional counterparts. Large solvent consumption is particularly concerning in a food analysis where complicated sample extraction and chromatography are routinely practiced. Supercritical fluid is a state of matter just like gas or liquid, and the characteristics of CO2 supercritical fluid are nonflammability, easy handling, and low toxicity, which are relevant to the operator safety. This webinar will show the characteristics of supercritical fluids CO2, the practicality of analytical techniques using it, and the applicability to GAC.
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Method development and Identification of Triacylglycerols species with SFC
Analyzing Triacylglycerols (TAGs) is essential to evaluate the quality and functionality of oils and fats. Chromatographic separation by typical methods that include GC and HPLC might be challenging due to a large number of TAGs species. Supercritical Fluid Chromatography (SFC) is one chromatography method that uses supercritical fluid (i.e. supercritical carbon dioxide) as a mobile phase. Here, we introduce the workflow of method scouting of SFC using Method Scouting Solutions and how to establish the optimized analytical method for TAGs species in natural oils and fats.
News / Events
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SFC Analytical Condition Parameters is available.
The approach for the SFC analytical condition development process is basically the same as for determining analytical conditions for HPLC.
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Latest issue of Shimadzu Journal, featuring Forensics / Toxicology, has come out.
This issue focuses on "Forensics / Toxicology", an area where science and technology are particularly important and needed. It starts with two customer interviews.
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Analysis of Tea Beverages Using Catechin Analysis Kit
The catechin analysis kit, developed in collaboration with the National Agriculture and Food Research Organization (NARO), provides validated test methods for catechins in tea leaves and green tea drinks from sample pre-treatment to HPLC measurement. You can accurately quantitate the content of 11 catechins (including methylated catechins) and caffeine in tea leaves. This article summarizes the results of the catechins quantitation in several beverages: green tea, black tea, and oolong tea.
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Extraction and Fractionation of Functional Ingredients with Online SFE-SFC
This article describes a preparative online supercritical fluid extraction-supercritical fluid chromatography (SFE-SFC) system that combines an extraction step using supercritical carbon dioxide as the solvent, a separation step using supercritical carbon dioxide as the mobile phase, and a fractionation step using a preparative-scale fraction collector (FRC).
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New UF-Amino Station
UF-Amino Station, which was developed in collaboration with Ajinomoto Co., Ltd., uses a special high-speed and high-separation column and the LCMS-2020 mass spectrometer, to perform batch analyses of 38* amino acids and amino acid-related components in a mere 9 minutes.
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What is Multidimensional Chromatography?
Multidimensional chromatography uses a combination of several chromatography techniques, separation modes, and columns to separate multiple components. It achieves significantly higher separation than normal one-dimensional chromatography.
Awards
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Pittcon Editors' Gold Award 2015
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2015 R&D magazine Award
Sustainability initiatives
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2 Zero hunger
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3 Good health and well-being
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7 Affordable and clean energy
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13 Climate action
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14 Life Below Water
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15 Life on land