Developing an optimal cell culture bioprocess for the production of biopharmaceuticals requires routine monitoring of medium conditions such as pH, dissolved gas, carbon source (glucose) and nitrogen source (glutamine) for optimization and control of the cell culture process. However, culture medium is composed of various other biologically important compounds such as vitamins, amino acids, nucleic acids and other primary metabolites. Comprehensive analysis of these compounds would lead to more detailed understanding of the bioprocess.