Post-Translational Modification Analysis | Phosphorylation Analysis by MALDI-TOF MS (3)

Post-Translational Modification Analysis

Phosphorylation Analysis by MALDI-TOF MS (3)

■ Phosphopeptide Enrichment Using TiO2

Phosphorylation of proteins is one type of post-translational modification. It is important in the control of biological functions. Recently, mass spectrometry has been applied to the analysis of phosphorylation sites. However, due to the low ratio of phosphorylation and the significant decrease in ionization efficiency due to phosphorylation, it is often difficult to analyze mixtures in their original state.
Phosphorylation analysis using a combination of TiO2-based phosphopeptide enrichment and MALDI-MS/MS (seamless PSD) is introduced below.
Fig. 1 shows the effect of enrichment by TiO2. The phosphopeptides were specifically enriched, and the phosphopeptide (m/z 1660) that could not be detected using desalting alone was observed.

Fig. 1 Phosphopeptide Enrichment Using TiO2: (A) Desalting by C18 Tip, (B) Enrichment by TiO2


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