Lipidomics

Lipidomics is a branch of metabolomics discipline and utilizes metabolic pathways of cellular lipids in biological systems. It is a comprehensive analysis of molecular lipid species and entails both quantitation and identification. Shimadzu offers both instruments and databases to ensure complete lipid profiling.

 

Development of an Analytical Tool Using Metabolic Maps Corresponding to 196 Eicosanoids and Related Fatty Acid Metabolites

Development of an Analytical Tool

Eicosanoids are lipid mediator species that are primarily part of the arachidonic acid cascade. The increasing sensitivity and high speed of LC/MS in recent years have led to the development of a method for comprehensively analyzing fatty acid metabolites of more than 100 targets, including not only arachidonic acid metabolites but also omega-3 fatty acid metabolites. This article introduces an example of the analysis of metabolites detected in human plasma and serum by a newly developed analytical tool using the metabolic map display of the target 196 eicosanoids and related fatty acid metabolites.

 

Comprehensive 2D Separation of Lipid Species in Mussels Using the Nexera-e System Combined with LCMS-IT-TOF Detection

Comprehensive 2D Separation of Lipid

Comprehensive two-dimensional (2D) liquid chromatography combines two independent separation modes. The different separation selectivity of the two dimensions are combined to improve the separation of components that are difficult to separate by single dimensional analysis.. This article presents an example separation of lipids in mussels using the Nexera-e, which is an effective system for the comprehensive separation of many lipid molecules.

 

Analysis of Eicosanoids in Blood Using the Same Sample Preparation Method as for Primary Metabolite Analysis

Analysis of Elcosanoids in Blood

Eicosanoids are considered important to the same extent as the primary metabolites in elucidating physiological function and searching for biomarkers. This study used derivatization including methoximation and trimethylsilylation to quantify seven eicosanoids: prostaglandin E2 (PGE2), 11β-prostaglandin E2 (11β-PGE2), 8-iso-prostaglandin E2 (8-iso-PGE2), 6-keto-prostaglandin F1α (6-keto-PGF1α), prostaglandin F2α (PGF2α), prostaglandin D2 (PGD2), and thromboxaneB2 (TXB2) using the GCMS-TQ8050NX.