LC/MS/MS Method Package for Bile Acids

For LabSolutions™ LCMS

LC/MS/MS Method Package Bile Acids Ver. 2


Ready-to-use method for analysis of 49 Bile Acids

Bile Acids Ver. 2

This Method Package allows comprehensive analysis of 49 Bile Acids from plasma, urine and feces. Bile Acids are essential for cholesterol metabolism and play a key role in absorption of fats in the small intestine. Additionally, they serve as signaling molecules that regulate metabolism. Their concentrations are used as markers of several diseases. Acute and chronic liver failure concern more than 12 million people worldwide, estimated by the World Health Organization, accounting for approximately 2 million deaths per year. Blood enzyme activity test (ALT, AST, etc.) and total bile acids assay (TBA) are widely conducted as examinations for the early diagnosis of liver diseases and liver function evaluation. More recently, monitoring of individual bile acids (IBA) was proposed for deeper pathologies characterization. However, bile acids wide structural diversity, associated with chemical similarities, leads to great challenges to analyze them.

This Method Package was developed to provide users a complete solution to perform routine analysis of selected primary, secondary and conjugates bile acids, in various matrices such as plasma, urine and feces.

Complete Solution from Sample Preparation to Result for Routine Analysis


  • Example pretreatment protocols (including automation) for plasma, urine and feces samples,
  • Robust chromatographic conditions for separation of isobaric species within a 10-min acquisition time,
  • Optimized MS/MS parameters for all targeted bile acids and their conjugates (up to 3 MRM transitions),
  • Optimized MS/MS parameters for 10 commercially available internal standards, covering all classes,
  • Ready to use method files for LCMS-8050/8060 and LCMS-8060NX,
  • Instruction manual for method installation.


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