LC/MS/MS MRM Library for Triglycerides
For LabSolutions™ LCMS

This MRM library targets fatty acids with carbon chain lengths of C14 to C22 and 0 to 6 unsaturation sites to profile the triglycerides (TG) in plasma. The library provides 195 MRM transitions, which enable 47 different triglycerides to be monitored. The MRMs were evaluated in human plasma in order to provide researchers a tool for qualitative profiling of fatty acid composition.
Example triglycerides with different fatty acid chains, corresponding to various MRM transitions in the library, are shown below. Fatty acid composition estimation is made possible by monitoring product ions from fatty acid neutral loss.

The MRM chromatogram below shows simultaneous analysis of 47 triglycerides in human plasma using Shimadzu’s LCMS-8060NX triple quadrupole mass spectrometer. MRM chromatograms corresponding to three typical triglycerides of unknown fatty acid composition are also shown. In the MRM chromatogram of TG 50:2, a doublet peak corresponding to TG 16:0_34:2 and three other peaks corresponding to TG 16:1_34:1, TG 18:1_32:1 and TG 18:2_32:0 can be observed. Assessment of this cumulative qualitative information shows that the TG 50:2 detected contains both TG 16:0 _16:1_18:1 and TG 16:0 _16:0 _18:2, demonstrating the utility of this qualitative MRM library.

Remarks and Precautions
LabSolutions LCMS Ver. 5.109 or later and LabSolutions Insight™ Ver. 3.8SP1 or later are required.
LabSolutions and LabSolutions Insight are trademarks of Shimadzu Corporation or its affiliated companies in Japan and/or other countries.
News / Events
-
Shimadzu has released the LCMS-2050 High-Performance Liquid Chromatograph Mass Spectrometer.
The LCMS-2050 single quadrupole mass spectrometer combines the user-friendliness of an LC detector with the excellent performance of MS to provide a complete package of easy to use high-level performance and compactness.
-
Shimadzu has released High Resolution Accurate Mass Library for Forensic Toxicology
The High Resolution Accurate Mass (HRAM) Library includes two MS/MS spectral libraries. Each library is built using two different separation conditions and is suitable for targeted or non-targeted screening of drugs of abuse, psychotropic drugs, pharmaceuticals, pesticides, and other compounds of toxicological interest.
-
What is a Method Package?
Method Package contains ready-to-use analytical conditions and sample pretreatment procedures optimized for Shimadzu mass spectrometers and specific analytical applications.
-
Shimadzu has released the LC/MS/MS Methid Package for Glycosaminoglycans
The study of Glycosaminoglycans (GAGs) or mucopolysaccharides, and enzymes involved in GAG metabolism is an important aspect of clinical research related to lysosomal storage. Quantitation of GAGs in bones, joints, the brain, heart, lungs, and other organs is a useful tool in this field of study. GAGs can be quantitated by measuring the sulfated disaccharides produced by enzymatic degradation of GAGs. The research group led by Hironori Kobayashi of Shimane University Hospital, Clinical Laboratory Division, has been instrumental in the development of methods for the detection of sulfated disaccharides by LC/MS.
-
Shimadzu has released the LC/MS/MS Method Package for PFAS in Drinking Water.
Provides procedures and methods for analyzing organofluorine compounds in drinking water according to EPA methods 533 and 537.1
-
Shimadzu has released the CoreFocus Library.
CoreFocus Library is a digital library where you can find and learn different methods for analyzing your target compounds.