ELSD-LT III - Applications

Evaporative Light Scattering Detector for HPLC

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UHPLC analysis of sugars

Because sugars only absorb very short wavelength UV light in the 190-195 nm range, they are typically measured with a refractive index detector. But gradient elution cannot be used, which makes simultaneous analysis very time-consuming.
Using the ELSD-LT III and a UHPLC column, gradient elution enables analysis time for a mixture of 5 sugars to be shortened from about 25 to 5 minutes.

Chromatogram of a standard solution of 5 sugars

Analysis of chondroitin

Chondroitin sulfate is a type of mucopolysaccharide that is commonly contained in eye medicines and supplements in the form of sodium chondroitin sulfate ester (often referred to as sodium chondroitin sulfate or SCS). Using gradient elution in reverse phase mode, excellent peak shapes can be obtained for quantitation in a short analysis time.

Analysis of chondroitin