Shim-pack WAX/WCX Series - Features

HPLC Column

Shim-pack WAX/WCX Series Analysis Examples

Analysis of Mononucleotides

■Peaks
  1. UMP
  2. CMP
  3. AMP
  4. GMP
  5. UDP
  6. CDP
  1. ADP
  2. UTP
  3. GDP
  4. CTP
  5. ATP
  6. GTP
■Conditions    
Column Shim-pack WAX-1 (50 mmL × 4.0 mmI.D., 3 μm)
(P/N: 228-16225-91)
Mobile Phase A) 20mM phosphate buffer solution (pH 7)
B) 480mM phosphoric acid buffer solution (pH 6.85)
gradient elution
Flow Rate 1.0 mL/min
Col. Temp. 45 °C
Detection UV 260 nm

Analysis of Protein Standard

■Peaks
  1. Ovalbumin
  2. Myoglobin
  3. α-Chymotrypsinogen A
  4. Ribonuclease A
  5. Lysozyme
■Conditions    
Column Shim-pack WCX-1 (50 mmL × 4.0 mmI.D., 5 μm)
(P/N: 228-16366-91)
Mobile Phase A) 20mM phosphate buffer solution (pH 6.0)
B) Sodium sulfate
gradient elution
Flow Rate 1.0 mL/min
Col. Temp. Ambient
Detection UV 415 nm

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Product Information

Column Stationary
phase
Particle
Size
(µm)
Dimensions
(Length × I.D.,mm)
P/N
Shim-pack WAX-1 Tertiary amino
group
3 50 × 4.0 228-16225-91
Shim-pack WAX-1 Tertiary amino
group
3 50 × 4.0 228-16225-92
Shim-pack WAX-1T Tertiary amino
group
3 50 × 4.0 228-18257-91
Shim-pack WAX-2 Tertiary amino
group
5 50 × 4.0 228-16365-91
Shim-pack WCX-1 Corboxyl group 5 50 × 4.0 228-16366-91
Pre-column Diol * Diol group 10 50 × 4.0 228-16367-91

  * Installed between the liquid pump and the sample injector to protect the column.