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Columns, Reagents and Consumables

Columns, Reagents and Consumables

Chromatography columns, reagents and consumables are vital pieces of equipment in laboratories. Shimadzu provides specialized high-performance liquid chromatography (HPLC) columnsgas chromatography (GC) columns, reagents and certified chromatography consumables are designed for reliable analytical results.

Shimadzu’s  chromatography columns are designed to ensure reliability, precision and adaptability across numerous industries and applications. The Shimadzu LC Columns Shim-pack column series has the capability to tackle a variety of particle sizes and analysis modes to ensure you have the optimum column for your application. These columns are suitable for small molecule pharmaceuticals, biopharmaceuticals, foods and beverage, environmental research, clinical research, as well as other industries. 

Column types include:

  • HPLC and UHPLC columns
  • Size-exclusion chromatography (SEC) columns for polymer, protein and biomolecule separation
  • Ion-exchange columns for complex sample purification
  • LC-MS compatible columns with low bleed and high sensitivity

Shimadzu’s essential reagents and consumables are designed to support consistency and reliability. The product range includes autosampler vials, syringe filters, solvents, buffers, and other reagents and consumables essential for laboratory efficiency and data integrity. 

FAQs

What core function do chromatography columns and consumables serve in lab analysis?

Chromatography columns are the engine room of analytical chemistry, providing the foundation for separation, identification and quantification of the molecular structure of sample mixtures. Reagents and consumables are the essential tools to make these analyses possible, comprising equipment including solvents, high-purity additives, vials and filters. By pairing columns with compatible reagents and consumables, you can optimize your equipment and processes for accuracy, precision and efficiency, while also benefiting the lifespan of the equipment itself.

How do different column types, like size exclusion chromatography column (SEC column) and ion-exchange columns, apply to diverse applications?

Different chromatography columns are better suited to certain sample types, which is why it’s important to understand the properties and complexities of both your sample material and the focus of your analyses. An ion-exchange column separates compounds based on differences in their net electrical charge, ideal for peptides or nucleic acids. An SEC column, on the other hand, separates molecules based purely on their molecular size, commonly used for characterizing polymers and proteins.

Why is the quality of reagents and consumables important for reliable chromatography results?

High-quality reagents and consumables are essential for prioritizing accuracy, sensitivity and efficiency in analytical chemistry. Low-quality reagents and consumables can introduce contaminants, which can, in turn, lead to ghost peaks, baseline drift and inconsistent results. By opting for high-purity solvents and Shimadzu-verified supplies, including glassware, you’re able to extend the lifespan of your invaluable chromatography column and optimize for the most accurate results across all analyses, including for mere trace-level particles. 

What are the key steps for protecting and extending the lifespan of a chromatography column?

Key steps for protecting and extending the lifespan of a chromatography column include:

  • Using a guard column to trap particulates and strongly retained compounds
  • Filtering all samples and mobile phases
  • Operating within Shimadzu’s (the manufacturer’s) specified limits for pressure, temperature, and pH.

What are the essential components for a liquid chromatography system, besides the instrument?

The essential components for a liquid chromatography system are the chromatography column, a pump, a sampler, the detector and the data processing device. The key consumables include the mobile phase (high-purity solvents/reagents), injection vials, and the connecting tubing and fittings, especially for LCMS chromatography workflows.

What are common chromatography consumables needed for routine analysis?

Common chromatography consumables needed for routine analysis include a continuous supply of autosampler vials and caps, syringe filters for sample pretreatment, pump seals, high-purity reagents and consumables for the mobile phase, and replacement filters for in-line filtration. These are the must-haves and it’s essential to use Shimadzu-verified supplies to ensure optimal performance.

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