Application of Evaporative Light Scattering Detector (Part 2)Analysis of Oligosaccharides in Beer

Introduction

The Evaporative Light Scattering Detector (ELSD-LT) is universal detector for HPLC. It makes a light scattering measurement using a photomultiplier of target analytes that have been dried of mobile phase through evaporation. It detects most non-volatile compounds in sensitivity depending on their mass. The ELSD-LT is particularly suited for such applications as sugars, fats and surfactans that have low absorbance and are difficult to detect using UV detector. This Application News introduces an example of analyzing oligosaccharides contained in beer, as an example of using the ELSD-LT for sugar analysis.

February 13, 2004 GMT