Lipid Mediator Profiling of Human Serum Using the Triple Quadrupole LC/MS/MS

Introduction

Lipid mediator is a generic term for bioactive lipids, which play a role in many biological functions. Recent development of a high sensitivity ultra-fast mass spectrometer enables lower detection limits for lipid mediator species. A comprehensive and highly sensitive application for the analysis of lipid mediators and their metabolites using a triple quadrupole mass spectrometer LCMS-8060 is presented here. Conditions developed for “LC/MS/MS Method Package for Lipid Mediators Ver. 2”, which can simultaneously analyze 158 lipid mediator-related compounds, were used as a basis for this application. Note that a single chromatographic analysis is capable of separating a wide range of species such as hydrophilic metabolites tetranor-PGs and hydrophobic arachidonic acid and both positive and negative ions are detected. Method sensitivity was compared between the LCMS- 8060 and a conventional LCMS-8050 system. For the LCMS-8060, signal intensity was around 3 times higher than that of the LCMS-8050.

June 5, 2015 GMT