Analysis of Water-Soluble Vitamins with Multi-Mode ODS Column Using LCMS-2020

Introduction

Dietary guidelines and nutritional supplements are a significant concern to health conscious consumers. Upper and lower limits of daily intake of such functional foods have been specified for 12 vitamins and 2 minerals. Water-soluble vitamins are one class of nutrients whose measurement is important to the food and nutritional supplement industries. Due to their high polarity, their retention is extremely weak when using reversed-phase chromatography. Historic use of ion-pair reagents when conducting LCMS analysis of such weakly retained analytes has resulted in reduced sensitivity. Here we present an example of analysis of 8 water- soluble vitamins using a cation exchange-anion exchange multi-mode ODS column, (Scherzo SM-C18, Imtakt Corporation) in conjunction with the LCMS2020 mass spectrometric detector. The gradient consisted of formic acid / ammonium formate buffer and acetonitrile mobile phases, components typical for high sensitivity reverse-phase LCMS analysis.

June 28, 2010 GMT