Fast Quantitative Analysis of Aminoglycoside Antibiotic Residues in Meat, Eggs and Milk and Identity Confirmation with MRM Spectrum Mode

Introduction

Aminoglycoside (AGs) are an antibiotic family widely used for the treatment of bacterial infections in cattle, sheep, pigs and poultry. They have a broad-spectrum activity and are used against Gram-positive and Gram- negative bacteria. AGs possess oto- and nephrotoxicity which did not hinder the widespread use of AGs in veterinary applications because of their low cost. Due to their high affinity for tissues, They may occur in meat, milk or eggs if the withholding period has not been observed or if used improperly. Therefore, eating food containing aminoglycosides can be potentially hazardous for human health. Regulatory agencies have set maximum residue limits (MRL) for these compounds with veterinary use. Aminoglycosides are very polar compounds poorly retained by reversed-phase liquid chromatography. Ion-pairing reagents are not desirable as they can easily contaminate the analytical system and interfere in other methods. A Method Package has been developed to overcome these problems. It comprises a protocol to generate clean extracts in a variety of commodities and a rapid quantitative method using hydrophilic interaction liquid chromatography (HILiC) combined with triple quadrupole mass spectrometry detection. When necessary, a second method for formal peak identification using MRM Spectrum Mode can be applied without changing reagents. In this document, we report the use of the method package to assess the safety level of several meat samples and milk.

May 25, 2018 GMT