Simultaneous Analysis of Amino Acids Using Automatic Pretreatment Function of ProminenceTM-i Integrated LC System

Introduction

The analysis of amino acids is necessary in various fields such as the fields of food and pharmaceuticals. A method often used in the analysis of amino acids with HPLC is post-column derivatization, and Shimadzu amino acid analysis system also uses the method. However, post-column derivatization is difficult to speed up due to the characteristics of the column which is used. Previously, in Application News No. L432A, we introduced the rapid pre-column derivatization method using a UHPLC system for amino acid analysis. This article introduces even easier and convenient method of amino acid analysis using an automated pre-column derivatization method which can be used with integrated HPLC system.

January 16, 2020 GMT