Simultaneous Analysis of Water-Soluble Vitamins by Newly Developed "Shim-pack MAqC-ODS Ⅰ" Column

Introduction

Vitamins play vital roles in the metabolic activities of the body. However, because they cannot be synthesized in sufficient quantities in the body, if at all, they must be ingested either in our meals or by other means, such as tablets. Vitamins can be classified into two groups, water- soluble and fat-soluble, and their analysis is generally conducted by high-performance liquid chromatography. Of the two types of vitamins, the water-soluble type consists mainly of highly polar basic components which exhibit weak retention in analysis by reversed-phase liquid chromatography. Since they exhibit weak retention, the analysis is typically conducted using ion- pair reagents. Using ion-pair reagents can be challenging because of the longer analysis time, widening of slow eluting peaks, difficulty in obtaining sufficient sensitivity and long equilibration times. Analysis efficiency is also compromised due to additional work-flow requirements, such as mobile phase preparation and column conditioning. Here, we introduce an example in which the newly developed "Shim-pack MAqC-ODSI" column is used to conduct simultaneous analysis of water-soluble vitamins without the addition of ion-pair reagents to the mobile phase.

August 1, 2014 GMT