Effect of Column Temperature on Organic Acid Separation

Introduction

Shimadzu's organic acid analysis system utilizes ion exclusion chromatography to first separate the target components, then introduce a pH buffer solution by the post-column method to adjust the pH to near neutral, and detect the organic acids using a conductivity detector to permit quantitation of the various organic acids detected. To increase the peak capacity for separations with large numbers of components, this system is often equipped with two columns connected in series. Although the temperatures of these columns are usually set to the same value, setting their temperatures to different values permits adjustment of the separation conditions to improve the resolution of closely eluting organic acids. Here we report the results of analysis of a mixture of organic acids using a dual-column, dual-temperature method.

February 18, 2013 GMT