A Novel Cell Culture Media Analysis Platform for Culture Process Development

Introduction

Optimization and control of cell culture processes are essential to increase production efficiency of biopharmaceuticals. In the field of cell therapy including regenerative medicines, enhanced control of the culture process is also becoming important to reduce cell variability and improve consistency of mass production of the cells. Comprehensive monitoring of culture supernatant components gives researchers useful information for these purposes. However, current technologies for process monitoring are limited to measurement of pH, dissolving gases, and some small compounds such as glucose, glutamine, lactate, and ammonia in culture supernatant. We have developed a “Cell Culture Media Analysis Platform, C2MAP system” that combines automated pretreatment module for culture supernatant samples with LC/MS/MS. This system can perform automated sample pretreatment and simultaneous analysis of up to 95 compounds including basal medium components and secreted metabolites (The list of target compounds is shown in the column to the right.). This system contains a software that can visualize temporal change in each culture supernatant components through the cell culture. In this application, we present features of the C2MAP system and its applications.

December 18, 2019 GMT