Autosampler
Nexera Series

SIL-30AC
SIL-30AC Autosampler
The total-volume injection SIL-30AC autosampler features a pressure tolerance of 130 MPa as well as the world’s fastest sample injection (10 seconds), which dramatically reduces the total cycle time. It includes auto pretreatment and overlapping functions as standard, and an optional loop-injection method configuration to minimize delay volume. With reduction of the needle contact area, special coatings, surface treatments, and a new needle seal, the SIL-30AC reaches a new level of low carryover performance, which is especially beneficial for LCMSMS analysis. In addition, the included sample cooler features a dehumidifier function for storing samples at a constant temperature between 4°C and 40°C.

Rackchanger II and SIL-30AC Autosampler
The rackchanger unit replaces the microplates on autosampler racks to enable samples to be continuously analyzed . It allows loading up to 12 microplates inside the unit (maximum 4608 samples). Samples can be temperature-controlled with a block heating/cooling system (with dehumidifier function and 4 °C to 40 °C range) not only to refrigerated temperatures, but also to stable temperature levels near room temperature.
Method | Variable sample injection volume and total volume direct injection |
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Allowable Operating Pressure | 66 MPa |
Injection Volume Range | 0.1 µL to 50 µL (0.1 µL to 100 µL with option) |
Number of Samples Processed | 175 (1-mL vials), 105 (1.5-mL vials, 70 for 20AC), 50 (4-mL vials), 192 (two 96-well MTP/DWP), 768 (two 384-well MTP/DWP) Besides these, ten 1.5-mL vials can be used |
Injection Volume Precision | Max RSD 0.3 % (normally max 0.2 %: depending on specified conditions) |
Cross-Contamination | Max 0.005 % (depending on specified conditions, without rinsing, normally max 0.0035 %) |
Needle Rinsing | Freely settable, before or after sample injection |
Operational Temp. Range | 4 - 35 °C |
Prominence Series
SIL-20A/20AC Autosampler

SIL-20A
The SIL-20A is a total volume injection type (direct injection) autosampler that enables high-speed injection and multi-analyte processing. Materials used in needle, valve, and other flow lines were closely considered and a needle rinsing function is provided to carefully minimize cross-contamination (carryover), which is especially useful for high-precision LC-MSMS analysis. The SIL-20AC includes a sample cooler that features a dehumidifier function for storing samples at a constant temperature between 4 °C and 40 °C.

Rack Changer
The rackchanger unit exchanges the microplates on autosampler racks to enable samples to be continuously analyzed. 12 plates can be mounted inside the main unit and stored under refrigerated conditions.
Method | Variable sample injection volume and total volume direct injection |
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Injection Volume Range | 0.1 µL to 100 µL (0.1 µL to 2000 µL with option) |
Number of Samples Processed | 175 (1-mL vials), 105 (1.5-mL vials), 50 (4-mL vials), 192 (two 96-well MTP/DWP), 768 (two 384-well MTP/DWP) *Twelve 96-well MTP/DWP in the rackchanger |
Injection Volume Precision | Max RSD 0.3 % (under specified conditions) |
Cross-Contamination | Max 0.005 % (naphthalene and chlorhexidine) |
Needle Rinsing | Freely settable, before or after sample injection |
Comparison for Autosampler Selection Autosamplers can be selected according to objectives.
Model Name | Features/Applications | Injection Volume (µL) | Number of Vials | Microplate Capacity | Main Liquid Contact Surface Materials |
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SIL-20A | Outstanding reproducibility and accuracy for trace volume injection. A needle rinsing function is included standard to minimize cross-contamination (carryover). It also allows high speed injection. (direct injection, specialized needle coating, and PEEK rotor) | 0.1 to 100 (max 2000) | 175 (1 mL), 105/70 (1.5 mL), 50 (4 mL) | Cools max two 96/384-well plates | PEEK, (SS) |

Manual Injectors
7725/7725i,
8125 (semi-micro),
9725/9725i (PEEK)
3725i (Preparative)
News / Events
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An Improvement in Preparative SFC Efficiency Utilizing Online SFE-SFC System as a “Solid Autosampler”
Preparative SFC is a groundbreaking separation and purification technology. It has a number of advantages in comparison to preparative LC including simplified posttreatment, shorter analysis times, and different separation selectivity. With preparative SFC, the injection of large sample volumes is often required in order to increase the recovered amount.
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SFC Analytical Condition Parameters is available.
The approach for the SFC analytical condition development process is basically the same as for determining analytical conditions for HPLC.
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Latest issue of Shimadzu Journal, featuring Forensics / Toxicology, has come out.
This issue focuses on "Forensics / Toxicology", an area where science and technology are particularly important and needed. It starts with two customer interviews.
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Analysis of Tea Beverages Using Catechin Analysis Kit
The catechin analysis kit, developed in collaboration with the National Agriculture and Food Research Organization (NARO), provides validated test methods for catechins in tea leaves and green tea drinks from sample pre-treatment to HPLC measurement. You can accurately quantitate the content of 11 catechins (including methylated catechins) and caffeine in tea leaves. This article summarizes the results of the catechins quantitation in several beverages: green tea, black tea, and oolong tea.
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Extraction and Fractionation of Functional Ingredients with Online SFE-SFC
This article describes a preparative online supercritical fluid extraction-supercritical fluid chromatography (SFE-SFC) system that combines an extraction step using supercritical carbon dioxide as the solvent, a separation step using supercritical carbon dioxide as the mobile phase, and a fractionation step using a preparative-scale fraction collector (FRC).
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New UF-Amino Station
UF-Amino Station, which was developed in collaboration with Ajinomoto Co., Ltd., uses a special high-speed and high-separation column and the LCMS-2020 mass spectrometer, to perform batch analyses of 38* amino acids and amino acid-related components in a mere 9 minutes.