LC-MS/MS Analysis Using Coinjection Method with the SIL-30AC

Introduction

Sample pretreatment takes time and introduces a potential for human error. For analyses that require sample pretreatment, automation of the pretreatment process is an effective means of improving throughput while maintaining repeatability. Automation also allows for analyses to be performed overnight, which is essential when screening a large number of samples. As well as a powerful rinse mechanism that dramatically reduces carryover, the Nexera Series SIL-30AC autosampler includes a pretreatment function as standard, so large volume samples can be analyzed in succession automatically with no operator intervention. Here we introduce the automation of an internal standard method using this pretreatment function along with the measurement results, for the simultaneous analysis of amino acids and acylcarnitines in a dried blood spot (DBS).

January 15, 2016 GMT