Determination of Molecular Mass and Quantification of Oligonucleotide Therapeutics Using Quadrupole Time-of-Flight Mass Spectrometer LCMS™-9030

Introduction

Oligonucleotide therapeutics are synthetic oligonucleotides that demonstrate their medical efficacy through binding to target genes or target proteins that may be responsible for a range of diseases. To date, eight types of oligonucleotide therapeutics have been approved, many of which have a length of approximately 20 bases. This article introduces an example of analysis using the Q-TOF mass spectrometer, LCMS-9030. As an oligonucleotide therapeutic, the 2’-MOE modified oligonucleotide having 20 bases was used. Accurate mass spectrometry determined the molecular mass of the therapeutic with an error of 3 mDa (0.05 ppm). When a calibration curve was prepared using the MRM mode on the LCMS-9030 mass spectrometer, linearity was observed within a range of 1 - 1000 ng/mL.

July 8, 2020 GMT