Thermal Stability Analysis of Nucleic Acid Drugs by TMSPC™-8 Tm Analysis System

Introduction

Functional nucleic acids, beginning with nucleic acid drugs, have attracted considerable attention in recent years. In the development and evaluation of these functional nucleic acids, it is essential to understand their thermal stability, as this is a factor that controls their structure and functions. Thermal stability analysis is also indispensable in techniques based on hybridization of nucleic acids such as PCR and DNA microarray. In this article, a thermal stability analysis (Tm analysis) of a nucleic acid was conducted using a Shimadzu UV-1900i ultraviolet-visible (UV-Vis) spectrophotometer and TMSPC-8 Tm analysis system. Simple calculation of the Tm value of nucleic acids is possible by using this system.

May 21, 2020 GMT