Analysis of Chloramphenicol in Shrimp and Chicken Egg Extracts Using Triple Quadrupole LC/MS/MS

Introduction

Chloramphenicol is an antibiotic with a broad antimicrobial spectrum and is widely used as a veterinary medicine for the prevention and treatment of livestock diseases. When the positive list system was introduced, chloramphenicol was set as a component that was not to be detectable in food. During the 2014 review, it could not be denied that it is genotoxic and possibly carcinogenic, so it was reevaluated as a component that should not have a set acceptable daily intake (ADI) which must not be contained in food continuously. In addition, since it has been confirmed that chloramphenicol glucuronide conjugates are hydrolyzed in vivo, generating chloramphenicol, the test method for chloramphenicol was revised in 2017 (Notification No. 49 of the Ministry of Health, Labour and Welfare, 2017), adding chloramphenicol glucuronide conjugates as a target of measurement. In this study, we present an example analysis of quantified chloramphenicol in shrimp and chicken eggs in accordance with the revised test method.

November 14, 2019 GMT