Determination of Molecular Weight of Synthetic Peptides by LC/MS Using Multi-Charged Ion Analysis Software

Introduction

Many natural poisons of animal origin contain peptides as their principle component, and the structural information of these peptides is utilized not only for investigating the mechanism of toxicity and addiction from the pharmacological point of view, but in basic research over a wide range of life sciences, including neuroscience and psychiatry. And, depending on their pharmacological possibilities, they may be used in the development of new drugs. A sufficient amount of sample is required to conduct activity measurement and functional analysis for peptide research, and for this, samples may be obtained via extraction from natural products, or depending on the case, they may be synthesized based on structural information. At this point, verification of sample synthesis and impurity analysis are required to ensure high-purity peptide synthesis. LC/MS, with its excellent sensitivity and qualitative performance, is widely used for peptide synthesis verification and impurity analysis. Here, Asp-His-Pro-Asn-Pro-Arg (DHPNPR), a synthetic product consisting of the N-terminal partial fragment of the C-type natriuretic peptide (OvCNP) that is present in platypus venom, was measured using the Nexera-i and LMCS-2020. Multi-Charged ion analysis software was then used to conduct molecular weight confirmation and impurity analysis. This Application News introduces the results of the analysis.

August 27, 2015 GMT