Application of Direct Analysis in Real Time (Part 2) Rapid Analysis of Triglycerides and Fatty Acids in Food Oil Using LCMS-2020

Introduction

DART (Direct Analysis in Real Time), when used with a mass spectrometer, permits quick analysis of analyte compounds without the need for sample pretreatment. Application News C108 introduced an analysis of free fatty acids and amino acids in food products using the LCMS-2020 equipped with DART as the ion source. Fatty acids present in foods are often bound to triglycerides, and when people ingest these triglycerides, not only do they serve as a source of energy, people acquire the physiological functions of the various fatty acids. Therefore, there is interest in research associated with triglyceride molecular species. Generally, LC and GC are used for triglyceride analysis, but these methods are known to have such drawbacks as complicated sample preparation, long analysis time and carryover. Here, using the same system as that used for the previous Application News, No. C108, we introduce an example of analysis of lipids in food containing triglycerides, without conducting any sample preparation.

August 25, 2015 GMT