Phospholipid analysis using SimLipid software

Introduction

Phospholipids (PLs) have a role of constituting a cellular membrane in a living cell and are also related to produce various fatty acids such as arachidonic acids, EPA and DHA which are precursors of bioactive lipids. Fluctuation of PLs concentration in a blood or a tissue is also known to be correlated with various disease. For example, Hyperlipidemia and arteriosclerosis are known to induce an elevation of lipid concentration in a blood and some nervous diseases are reported to change the ratio of fatty acid constitution of phospholipids. Thus although phospholipids are reported to be related with various disease, a number of phospholipid species is enormous. PLs are classified to glycerophospholipid and sphingophospholipid by the structural body. Furthermore, PLs are classified to PC, PE, PG, PI, PS, PA and SM by its characteristic head group. These PLs have diverse fatty acids different in a length of carbon chain, an number of double-bond. Here we shows the analyzing results by LCMS-8060 of phospholipid changes in a liver tissue between a control and a mouse which a fluorescent probe has been administered by a tail vein injection. In this analysis, SimLipid software from PREMIER Biosoft, USA (www.premierbiosoft.com) was used to estimate the candidate of PLs fluctuated between a control and a probe administered mouse.

April 3, 2017 GMT