Qualitative Determination of Genetically Modified Bt-11 Corn Using the MultiNA

Introduction

A plant genome extraction reagent kit was used to extract the genome of the genetically modified Bt-11 corn standard, using the corn endogenous Zein gene as an internal reference. Specific primers were designed for the Bt-11 transgene sequence for PCR amplification, and the Shimadzu MultiNA microchip electrophoresis system was used to determine the amplified product. The results indicate that the fragment length of the amplified product is largely consistent with the theoretical length, indicating that the Bt- 11 transgene is contained. This experiment indicates that the qualitative determination of Bt-11 in genetically modified corn can be realized using the MultiNA.

November 5, 2014 GMT