Qualitative Determination of Edible Corn Oil Using the MultiNA and the PCR Method

Introduction

Currently, edible oil adulteration is a common practice. This not only affects the physical health of consumers, but also lowers their confidence and has a serious impact on their interests. This paper proposes the design of a PCR primer for the specific gene of the species to be identified by DNA- based molecular biological means because different species have different DNA sequences, and determines the existence and chain length of the PCR amplified product using the MultiNA, thereby establishing a MultiNA-based method for edible oil identification. The specific gene PCR extracted from corn oil was amplified, and the size of its amplified product as determined by the MultiNA was 196 bp, largely consistent with the size of the PCR target product of the corn gene (190 bp). The experimental results indicate that this method can realize the qualitative determination of edible oils.

November 5, 2014 GMT