Phosphorylation Analysis by MALDI-TOF MS (1) Measurement Mode and Matrix Selection for Phosphopeptide Detection

Introduction

Phosphorylation is one type of protein post- translational modification (PTM) that plays a significant role in important biological functions. Mass spectrometry is recently being applied in phosphorylation interrogation, and various results have been reported. In almost all cases, however, the instrument configuration used has been based on electrospray ionization (ESI). Here we introduce a method useful for the detection of phosphopeptides by MALDI technique.

July 8, 2009 GMT