Liquid Sample Analysis with the MIRacle Single-Reflection ATR Accessory

Introduction

This Application News presents examples of liquid-sample analysis using the MiRacle single-reflection ATR accessory that was introduced in Application News A304. The liquid-film method and multiple-reflection ATR (horizontal ATR) method are frequently used for liquid-sample analysis. Each method offers its own merit: the liquid-film method allows adjustment of the absorption intensity (sensitivity) with the cell thickness; and the multiple-reflection ATR method allows easy cleaning after sampling analysis. However, neither of these methods can achieve measurement without peak saturation across the full wavelength range: the liquid-film method requires adjustment of the cell thickness and the multiple-reflection method requires adjustment of the drip-volume (contact area onto the prism. Conversely, as only one reflection is required using the MIRacle single-reflection ATR accessory, good spectra can be obtained without peak saturation simply by dripping the sample onto the prism.

May 9, 2002 GMT