Identification of Rice Varieties by Multiplex-PCR with MCE-202 "MultiNA"

Introduction

Rice is one of Japan's agricultural products for which package labeling of the variety name and production region are obligatory according to the Japanese Agricultural Standard. However, since mislabeling can be problematic, various technologies have been developed to scientifically verify label content. One method that is widely used for assessing the rice variety is to amplify a specific gene region from extracted rice DNA using PCR, and then to analyze the PCR product using an electrophoresis analyzer. Here we introduce a method of conducting multiplex PCR to identify rice varieties using an identification kit capable of distinguishing among 112 commercially available varieties from DNA extracted from polished rice. The electrophoresis patterns of the obtained PCR products were determined using the MCE-202 "MultiNA" Microchip Electrophoresis System.

October 22, 2010 GMT