Fractionation by Preparative LC-MS and Fraction Verification byUltra Fast LC-MS

Introduction

Mass-directed fractionation is a method based on information obtained from the mass spectrometer. Here, mass spectrometric analysis is conducted for the constituents separated by the column, and fractionation is conducted using the mass chromatogram intensity and slope as indicators. The high LC separation performance coupled with the high MS qualitative analysis performance ensures that the target constituents will be collected with a high degree of certainty. Furthermore, purity confirmation of the collected fractions is possible by subjecting the fractions to LC- MS analysis. In this case, when large numbers of fractions are obtained, optimization of the purification and fractionation conditions can be conducted with greater efficiency through high-speed analysis. This Application News introduces an example of LC- MS fractionation of catechins in green tea, as well as an example of purity confirmation using ultra fast analysis by a high-throughput LC-MS system coupled with the Prominence UFLC.

February 13, 2008 GMT