Fast LC/MS/MS Method for Quantitative Determination of Omeprazole in Human Plasma

Introduction

A sensitive and a fast LC/MS/MS method for quantification of Omeprazole in human plasma using UHPLC NEXERA coupled to LCMS-8040 Triple Quadrupole Mass Spectrometer was described. A simple liquid-liquid extraction method for extraction of Omeprazole and internal standard (Tolbutamide) from the biological matrix was employed. The new advanced LCMS-8040 enabled the quantification of Omeprazole from human plasma samples over a concentration range of 1.0 (LLOQ) to 2000ng/mL. The constructed calibration curve was linear with a regression of >0.99.

February 6, 2014 GMT