Detection of Mold and Yeast Genes with MCE-202 MultiNA

Introduction

Mold and yeast exist throughout the environment and there are many beneficial varieties of each in food sources such as miso (fermented soybean paste), cheese, bread, wine, and sake, etc., and those that are mildly to extremely toxic. Polymerase Chain Reaction (PCR) technology has been used in recent years as one of the molecular biological techniques for detecting fungi such as molds and yeasts. When PCR is performed, a complicated pretreatment operation is typically required to extract the DNA from fungus samples like molds and yeasts. Here we introduce an example of direct mold and yeast analysis using the MCE-202 “MultiNA” DNA/RNA analyzer based on microchip electrophoresis technology, in which the PCR reagent “Ampdirect Plus” is used to enable the PCR reaction without the need for any PCR pre-processing operations.

June 28, 2010 GMT