Detection of Food Poisoning-Related Genes with MCE-202 MultiNA

Introduction

In recent years, genetic detection methods have become widely adopted for the identification of the causative agents of food poisoning, allergies, and infectious diseases such as influenza. The most widespread conventional gene-level detection method involves amplification of specific genes using PCR (Polymerase Chain Reaction), followed by detection of the amplification products and sizes using electrophoresis. The conventional agarose gel electrophoresis technique is a labor-intensive series of processes from preparation of the gel to obtaining results. Furthermore, size measurement involves visual comparison with the sizes of known bands, which often leads to variation in results due to the reliance on individual objectivity. Here we introduce the analysis of genes related to food poisoning using the MCE-202 MultiNA Microchip Electrophoresis System for DNA/RNA Analysis. This system offers high-speed, automated analysis, higher detection sensitivity than agarose gel electrophoresis, and automatically calculated size estimation using pretreatment and electrophoretic parallel processing.

October 16, 2014 GMT